Heme was preincubated for 5 min with A when added before the metals. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. 0% and ± 0. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. Create custom fluorescence assays without having to purchase expensive filter sets. Briefly, cells were seeded into a. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Binding of capture antibodies to polystyrene beads and biotinylation. 6 g sodium acetate trihydrate and 2. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). DNA extraction from blood meal ELISA homogenate. SpectraMax QuickDrop Micro-Volume Spectrophotometer. AoSMCs were seeded into 6-well culture plates. 3: The cuvette chamber. 8. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Awareness Technology Inc. Molecular Devices SpectraMax Gemini XS Microplate Reader. The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. MMP‑1 inhibition assay. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). 5. Calculating % of total mediator release in RBL assay. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . D. NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. VersaMax reads 96-well and strip-well plates. Molecular Devices Spectramax 340PC instructions. The ABS reader ranges from. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. The VersaMax and SpectraMax 190 read 96-well microplates. , Sunnyvale, CA, USA) at 595 nm. Although not statistically significant, pain was reduced by 2 NRS steps or more during supplemental treatment with naloxone in 36% of subjects when. 30 µs. Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. 5. SpectraMax Plus 384 measuring instruments pdf manual download. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). Inter-assay variation was prevented by testing all of the samples on the same day. Nitrocefin hydrolysis was monitored at 482 nm on a SpectraMax 340 UV-visible plate reader for high-throughput assays and an HP8453 UV-visible spectrophotometer for the low-throughput assays. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Microplate Spectrophotometer Operator’s Manual. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. The assays contained 0. . The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. Statistical analysis. The sensitivities of the hormones are Prolactin: 0. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). 30. 3 mm. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. Dissolve 13. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. mums. Software (SoftMax Pro ® software or comparable plate reader software). SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. The inhibition percentage was determined by linear regression through ranges of 20 to 30. gp70 blocking. The initial rate of hydrolysis was determined using the early linear region of. SpectraMAX 340. Revvity. Molecular Devices Spectramax 340 Plate Reader Repair. or microplate reader (e. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. SPECTRAmax® 190. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. 5. After adding sample, the mixture was incubated for 30 min at 37°C, with one unit of enzymatic activity being defined as an increase in absorbance of 0. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance platereader (Molecular Devices, Union City, CA, United States). Details. plates can be used in the SPECTRAmax PLUS 384. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. 190–850 nm <± 1. Includes: Computer Loaded with Softmax Pro 5. ). All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). 2. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. TDP-43 stress granular cells were treated with 70% ice-cold. When necessary, samples were. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Acceptor: 665nm. control. Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). \r plates can be used in the SPECTRAmax PLUS 384. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Six wells were assayed for each set of conditions and stadard deviations. Lactate Dehydrogenase (LDH) Release. SPECTRAmax® 190. These lab workhorses are used in drug discovery, research, bioassay validation, and. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 94 g calcium chloride dihydrate (both. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 1x10 5 transduced, GFP + SK-NEP-1. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Samples containing 25 μg of protein were separated on 12. Or scan up to 384 wells at any time to determine the best assay conditions. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. Versteegh, Yoran H. Products : SpectraMax Plus 384 reader,. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. 2 0. The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. , Sunnyvale, CA). 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. 5. On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. One plate carrier adapter is provided with the instrument. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). , Sunnyvale, CA, USA) at a wavelength of 450 nm. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). 30. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. About GMI provides new and GMI Certified Pre-Owned scientific instrumentation and service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics and analytical laboratories, in the United. 4) was followed according to Vanacker et al. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. Polystyrene plates (384-well) were precoated overnight at 4°C with 2 μg/ml of murine, human, or chimeric RANKL. Donor filter: 620 nm. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Quick View. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 312 gain calibration failed. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. 5 stars - 1602 reviewsThis assay typically develops in 1. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 00 USD. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. Call 1-800-745-2710. SpectraMax Multi-Mode Microplate Readers. Donor: 620 nm. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. California Lightworks uses only the highest quality LEDs from Osram. Data are expressed as percent of maximum binding for each protein. 306 plate air cal fail,low light. ELISA plates were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Figure 1. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. They are for reference only. Intra- and inter-assay coefficients of variation were lower than 8. The absorbances were transformed to velocity of substrate consumption (nmol/min). This reader only allows high performance HTRF measurement when assays are run in. Absorbance was read at 450 nm using a Molecular Device Spectramax 340. Softmax PRO v5. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). 4; 100 m M NaCl, 0. density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). Spectral Range. Both readers feature an 8-channel read head for fast reads and. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 4. Samples were plated in duplicate (r=0. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. Protein electrophoresis system (BioRad, Hercules, CA) and Odyssey Infrared Imaging System (Li-Cor Biosciences, Lincoln, NE) were used in western blot. Wound healing assay. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). 5. The SpectraMax® iD3 Multi Mode Microplate . The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. ). used. SpectraMax Gemini EM & FLEXstation (bottom read) 1. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 10. Donor filter: 616/10 nm. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Crowder, Ziqian Zeng, Alissa N. The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. 4) was followed according to Vanacker et al. Up for your consideration is a Molecular Devices SpectraMax 340 Microplate Spectrophotometer Model# 340 Condition is for parts or repair. • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. The reaction was stopped with 1 M hydrochloric acid after 15 min. The concentration of TNFα in the experimental samples was calculated from a mouse TNFα standard curve of 15–2000 pg/ml. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Recalcification (clotting) time was determined by monitoring absorbance at 650 nm at 10-sec intervals in a SpectraMax 340 microtiter plate reader, with onset time (the time to a linear increase in the OD, which reflects the maximal rate of formation of insoluble fibrin) set at an OD of 0. The light features a slim profile to prevent. The plate was covered and incubated for one hour at room temperature. Read one sample or up to 384. Absorbance at 490 nm was determined using a SPECTRAmax 340 (Molecular Devices Corporation). The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Summary of Contents for Molecular Devices SPECTRAmax 340PC. 1 mL of acetylacetone. Detection limits. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. 1: SpectraMax Plus 384 components. Molecular Devices Corporation 1311. control or celatrol-treated cells. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San Jose, CA, USA). BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hIranian Journal of Basic Medical Sciences ijbms. 0. Near bottom. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. Furthermore, the stain was extracted using 10% acetic acid, and its absorbance was measured at 595 nm in a microtiter plate reader (SpectraMax 340; Molecular Devices). MOLECULAR DEVICE M5. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Includes: SpectraMax 340PC384. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. Each experiment was performed in duplicate. Molecular Devices SpectraMax Gemini XS Microplate Reader. G. Streamline GLP/GMP compliance. SpectraMax Multi-Mode Microplate Readers. the SpectraMax iD3 is a fantastic plate reader for a. Microplate Spectrophotometers. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. The fluorescence intensity was measured using the SpectraMax 340 microplate reader (Molecular Devices) at the following conditions: excitation at 475 nm and emission at 529 nm (green fluorescence) or 590 nm (red fluorescence). The absorbance was measured at 340 nm for 2 min at a 15 s time interval. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. 4. The reaction mixture was incubated for 5 min. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. (1993). According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Italic and boldface type are used for emphasis. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). , Sunnyvale, Calif. Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. The cell survival. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . 1% N-1-naphtylethylenediamine dihydrochloride, 2. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. Unit powers. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. To measure the cell biomass (g/L),. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. plates can be used in the SPECTRAmax PLUS 384. Cell culture 3T3 fibroblasts and RAW 264. Manager: Yinghua Chen, PhD Contact Email: yinghua. Immunofluorescence and Aggregation Analysis. albicans pre. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. 3. Each SpectraMax® 340 PC 384 Microplate Reader has an. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The cell proliferation on different Ti discs was assessed by measuring the absorbance at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Devices, Sunnyvale, CA). SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Details. SpectraMax Multi-Mode Microplate Readers. 7. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. 5mM), and changes in OD 405 nm. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. , San Jose, CA) using Softmax Pro 7 software. Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. The activity of glutathione peroxidase (GPX) was quantified spectrophotometrically at 340 nm, following the method of Takeda et al. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). One plate carrier adapter is provided with the instrument. The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Gerritsma,B. 10× trypsin 0. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. 04. Near bottom. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). Acceptor: 665/10 nm. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. Details. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. PathCheck SensorThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. The absorbance at 405 nm was determined using a microplate reader (spectraMAX 340). 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. SpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. Labsystems Luminoskan RT Microplate Reader. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. VersaMax reads 96-well and strip-well plates. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Stat Fax 3200 Microplate Reader. SpectraMax 190 reads 96-well and strip-well plates. 2 Chemical Reagents 1. 20. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. Optical density was read at 405 nm after 30 min using a Molecular Devices SpectraMax 340 spectrophotometer. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 4 0112-0126D SafetyInformation. Binding affinity for denosumab. 0 OD. Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. 2. Alkaline phosphatase (ALP) activity. Cross sections of mouse skin was prepared and stained as previously described . SpectraMAX 340. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 4. using SpectraMax i3 (Molecular devices, San Jose, USA). 3 0. 340 nm. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. 340 nm / 70 nm. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. , Sunnyvale, CA). Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. These models can no longer be repaired. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). Photometric Range: 0. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass.